FastQCFastQC Report
mar. 7 juin 2016
CDM13.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCDM13.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28248
Sequences flagged as poor quality0
Sequence length249-470
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCGATGAAGAACGCAGCGAAATGCGATACCTAGTGTGAATTGCAGCC876431.025205324270743No Hit
GCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATATGAATTGCAGAT412414.599263664684226No Hit
CTTCCGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTG373213.211554800339847No Hit
GCATCGATGAAGAACGCAGCGAATTGCGATATGTATTGTGAATTGCAGAT24418.64131973945058No Hit
TTCCGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGA10453.699376947040498No Hit
TCTTCCGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGT7562.6762956669498723No Hit
GCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAA6412.269187199093741No Hit
TCCGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGAC5401.9116397621070518No Hit
CCGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGACT3601.274426508071368No Hit
CGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGACTT2360.8354573775134523No Hit
GCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGACTTGCAGAT1060.37524780515434725No Hit
GATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGACTTG800.2832058906825262No Hit
GCATCGATGAAGAACGCAGCGAATTGCGATACCTAGTGTGAATTGCAGCC720.2548853016142736No Hit
GCATCGATGAAGAACGCAGCGAATTGCGATAAGTAATATGAATTGCAGAT560.19824412347776835No Hit
TGCTGCCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCAATGTGGCCGA480.16992353440951571No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACCTAGTGTGAATTGCAGCCA380.13452279807419995No Hit
GCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAAAT370.13098272444066836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAT50.00770321027.9248370-379
TCAAACT305.313769E-4342.6416370-379
GAGCACA204.3755335E-6333.43314260-269
CTCTGCA106.8993046E-4315.30327
CATGAAG106.8993046E-4315.30322
GCCTCCC155.493748E-6315.30325
CTCCCGT155.493748E-6315.30327
GCGATGA106.8993046E-4315.30321
TTCCGCT106.8993046E-4315.30322
CACGCAG106.8993046E-4315.30329
GAAGCAC106.8993046E-4315.30325
GAAGTAC106.8993046E-4315.30329
ATGAAGT106.8993046E-4315.30327
ATGAAGC106.8993046E-4315.30323
GAGCTGC106.8993046E-4315.30322
TGCCTCC155.493748E-6315.30324
CCCGTAG155.493748E-6315.30329
CCTCCCG155.493748E-6315.30326
GCTGCCT155.493748E-6315.30322
GCTGCAT106.8993046E-4315.30324
Produced by FastQC (version 0.11.3)