FastQCFastQC Report
mar. 7 juin 2016
CDP13.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCDP13.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27167
Sequences flagged as poor quality0
Sequence length250-481
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATATGAATTGCAGAT730826.900283432105127No Hit
GCATCGATGAAGAACGCAGCGAAATGCGATACCTAGTGTGAATTGCAGCC446516.43538116096735No Hit
GCATCGATGAAGAACGCAGCGAATTGCGATATGTATTGTGAATTGCAGAT27039.949571170905879No Hit
CTTCCGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTG18126.669856811572863No Hit
GCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAA14085.182758493760812No Hit
GCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAAAT10904.012220708948357No Hit
GCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAA10173.7435123495417235No Hit
TTCCGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGA4241.5607170464166085No Hit
TCTTCCGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGT3501.2883277505797475No Hit
TCCGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGAC2380.8760628703942283No Hit
CCGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGACT1560.5742260831155446No Hit
TGCTGCCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCAATGTGGCCGA1350.4969264180807597No Hit
GCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCACAT1060.3901792616041521No Hit
CGATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGACTT990.36441270659255715No Hit
GCATCGATGAAGAACGCAGCGAATTGCGATAAGTAATATGAATTGCAGAT560.2061324400927596No Hit
GCATCGATGAAGTACGCAGCGAAATGCGATAAGTAATATGAATTGCAGAT460.1693230757904811No Hit
GCATCGATGAAGAACGCAGCGAATTGCGATACCTAGTGTGAATTGCAGCC360.1325137114882026No Hit
GCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGACTTGCAGAT340.1251518386277469No Hit
GATCTGCATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGACTTG330.12147090219751906No Hit
CATCGATGAAGAACGCAGCGAAATGCGATAAGTAATATGAATTGCAGATT290.10674715647660765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAG100.0073275951051.0563390-399
CGGCTTT1300.0597.4763350-359
AAGAAGC1557.070412E-9438.91614420-428
GAAGTAC105.288557E-4344.545149
CACCGAT105.288557E-4344.545142
GAAAAAC105.288557E-4344.545149
ACCGATG105.288557E-4344.545143
GATGAAA202.7997885E-8344.545146
GCTGCCT252.0372681E-10344.545142
GGAAGAA105.288557E-4344.545146
GCACCGA105.288557E-4344.545141
GCCTCCC301.8189894E-12344.54515
CTCCCGT301.8189894E-12344.54517
CCCGTAG301.8189894E-12344.54519
CCTCCCG301.8189894E-12344.54516
CTGCCTC301.8189894E-12344.54513
GCATCGG153.8538765E-6344.54511
TGCCTCC353.6379788E-12295.32444
TCCCGTA353.6379788E-12295.32448
GCATCGA24750.0289.557131
Produced by FastQC (version 0.11.3)