FastQCFastQC Report
mer. 20 déc. 2017
MC2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60000
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCAGGGTATCTAATCCTGCTGCCTTCCGTAGATCGGAAGAGCGTCGTG1094918.248333333333335No Hit
TACCAGGGTATCTAATCCTGCTGCCTCCCGTAGATCGGAAGAGCGTCGTG1060117.668333333333333No Hit
TACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGT56429.403333333333332No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGT45907.6499999999999995No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGT45857.641666666666666No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGT33825.636666666666667No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGT28564.760000000000001No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGT23553.925No Hit
TACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAACGT20853.4750000000000005No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGT16432.7383333333333333No Hit
TACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAGTGT8911.485No Hit
TACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAACCTCAGCGT6981.1633333333333333No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGT2250.375No Hit
TACCAGGGTATCTAATCCTGCTGGCTCCCGTAGATCGGAAGAGCGTCGTG1390.23166666666666666No Hit
TACCAGGGTATCTAATCCTGCTGGCTTCCGTAGATCGGAAGAGCGTCGTG1290.215No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTGAGTGT1230.20500000000000002No Hit
TACCAGGGTATCTAATCCTGCTGCCTTCCGTAGATCGGAAGACCGTCGTG1070.17833333333333334No Hit
TACCAGGGTATCTAATCCTGCTGCCTCCCGTAGATCGGAAGACCGTCGTG1010.16833333333333333No Hit
TACCAGGGTATCTAATCCTGCTGTCTTCCGTAGATCGGAAGAGCGTCGTG800.13333333333333333No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAGCGT740.12333333333333332No Hit
TACCAGGGTATCTAATCCTGCTGTCTCCCGTAGATCGGAAGAGCGTCGTG720.12No Hit
TACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGT690.11499999999999999No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTT100.0014761966244.8075245
CCGGCCA151.5169759E-5244.8075245
CCGGATA600.0244.8075245
CCGGACC100.0014761966244.8075245
CGGGACA251.6025297E-9244.8075245
CGTTTGT100.0014761966244.8075245
CCTGATT201.5556179E-7244.8075245
TACCGGG151.5169759E-5244.80751
ACCAGGG58550.0244.180312
TACCAGG58600.0243.971981
AGGGTAT58550.0243.971255
CCAGGGT58650.0243.555273
CAGGGTA58650.0243.555274
GGGTATC58600.0243.554216
GGTATCT58600.0243.136467
TATCTAA58800.0242.517649
GTATCTA59200.0240.879018
CCGTTTT1000.0220.32675245
CCGGACA3750.0218.6947245
CCTATTT1400.0218.57812245
Produced by FastQC (version 0.11.5)