The BSFA project

The 17 partners of the European functional analysis project of Bacillus subtilis have disrupted 1146 unknown genes, out of the 4100 identified on the chromosome of the bacterium. You will find in this site three kinds of results : GROWTH CURVES OF STRAINS in Rich and Minimal medium REPORTER GENE ACTIVITY of STRAINS SYSTEMATIC DETERMINATION OF PHENOTYPE (which is positive for 543 genes)

Protocols, phenotypes and laboratories

Class code Metabolic class Prot. code Protocol designation Coordinator Laboratory   1   Metabolism of small molecules and inorganics   1   Metabolism of small molecules and inorganics 10    Tests with nitrogen or carbon sources   Aymerich S.   INRA GMC   1   Metabolism of small molecules and inorganics 24    Amino-acids analogs and Translation   Rapoport G.   IP   1.1   Carbon metabolism 15    Glucose effect   Rapoport G.   IP   1.2   Nitrogen and sulphate metabolism 14    Induction / repression by amino acids   Rapoport G.   IP   2   Macromolecule Metabolism   2   Macromolecule metabolism 12    Shift down / Reversion frequency / Incorporation   Ehrlich S.D.   INRA GMC   2.1   DNA metabolism 8    Genetic recombination   Bron S.   RUG   2.3   Protein metabolism and secretion 7    Protein secretion tests   Bron S.   RUG   3   Cell structures and mobility   3.1   Cell envelope 17    Lysozyme sensitivity tests   Harwood C.   UNEW   3.1   Cell envelope 18    Test for alkaline phosphatase and betagalactosidase on low and high phosphate media   Harwood C.   UNEW   3.2   Motility 5    Motility tests   Galizzi A.   UNIPAV   4   Stress and stationary phase   4.1   Stress analysis 6    Oxidative stress   Aymerich S.   INRA GMC   4.1   Stress analysis 16    Salt stress by sodium chloride   Rapoport G.   IP   4.1   Stress analysis 11    Temperature and pH stress   Schumann W.   UBT   4.1   Stress analysis 25    Metals   Rapoport G.   IP   4.1   Stress analysis 23    Electron transfert   Rapoport G.   IP   4.1   Stress analysis 22    Metals chelators   Rapoport G.   IP   4.1   Stress analysis 21    General stress   Rapoport G.   IP   4.1   Stress analysis 19    Stress and starvation tests   Hecker M.   EMAUG   4.2   Stationary phase 1    Stationary phase : Calcium / EGTA sensitivity   Seror S.   UPXI   5   Cell processes   5   Cell processes 4    Tests for cell cycle and sporulation defects   Errington J.   U.OXF.DSP   5.1   Cell cycle 2    Cell cycle tests   Seror S.   UPXI   5.2   Competence 9    Transformability tests   Bron S.   RUG   5.3   Sporulation 3    Heat / Chloroform resistance of spores   Moir A., Foster S.   U.Sheffield   5.3   Sporulation 13    Heat tests and morphology studies on spores   Stragier P.   CNRS Paris-B   5.4   Germination 20    Germination tests   Moir A., Foster S.   U.Sheffield