[xml] [original]

Header

Title

Mutagenesis of both prophenoloxidases in the fall armyworm induces major defects in metamorphosis - PubMed

Authors

Magali Eychenne 1

Availability

Better title
Mutagenesis of both prophenoloxidases in the fall armyworm induces major defects in metamorphosis - PubMed
Source
PubMed (nih.gov)
URL
https://pubmed.ncbi.nlm.nih.gov/35568240/?utm_source=FeedFetcher&utm_medium=rss&utm_campaign=None&utm_content=1DAyVQqGr_VN4_17R7mUPjsF8UnGxmBKs4upqQvj5say1q_9Y7&fc=None&ff=20220516224254&v=2.17.6
Date
2022-01-01
Description
Abstract
Upon infection, the phenoloxidase system in arthropods is rapidly mobilized and constitutes a major defense system against invaders. The activation of the key enzymes prophenoloxidase (PPO) and their action in immunity through melanization and encapsulation of foreign bodies in hemolymph has been de …
Keywords
tags = pmid:35568240, doi:10.1016/j.jinsphys.2022.104399, Magali Eychenne, Pierre-Alain Girard, Nicolas Nègre, Animals, Catechol Oxidase, Enzyme Precursors* / genetics, Larva, Monophenol Monooxygenase* / genetics, Mutagenesis, Spodoptera / genetics, PubMed Abstract, NIH, NLM, NCBI, National Institutes of Health, National Center for Biotechnology Information, National Library of Medicine, MEDLINE
Body

Mutagenesis of both prophenoloxidases in the fall armyworm induces major defects in metamorphosis

PMID:
35568240
DOI: 10.1016/j.jinsphys.2022.104399

Mutagenesis of both prophenoloxidases in the fall armyworm induces major defects in metamorphosis

Abstract

Upon infection, the phenoloxidase system in arthropods is rapidly mobilized and constitutes a major defense system against invaders. The activation of the key enzymes prophenoloxidase (PPO) and their action in immunity through melanization and encapsulation of foreign bodies in hemolymph has been described in many insects. On the other hand, little is known about PPOs involvement in other essential functions related to insect development. In this paper, we investigated the function of the two PPOs of the crop pest, Spodoptera frugiperda (PPO1 and PPO2). We show that PPOs are mainly expressed in hemocytes with the PPO2 expressed at higher levels than the PPO1. In addition, these two genes are expressed in the same tissue and at the same stages of insect development. Through the generation of loss-of-function mutants by CRISPR/Cas9 method, we show that the presence of PPOs is essential for the normal development of the pupa and the survival of the insect.

Keywords: CRISPR; Development; Fall armyworm; Lepidoptera; Melanization; Phenoloxidase; Spodoptera frugiperda.

Copyright © 2022 Elsevier Ltd. All rights reserved.

Similar articles

Drosophila innate immunity: regional and functional specialization of prophenoloxidases.BMC Biol. 2015 Oct 1;13:81. doi: 10.1186/s12915-015-0193-6. BMC Biol. 2015. PMID: 26437768 Free PMC article.

Prophenoloxidase activation is required for survival to microbial infections in Drosophila.PLoS Pathog. 2014 May 1;10(5):e1004067. doi: 10.1371/journal.ppat.1004067. eCollection 2014 May. PLoS Pathog. 2014. PMID: 24788090 Free PMC article.

Prophenoloxidase genes and antimicrobial host defense of the model beetle, Tribolium castaneum.J Invertebr Pathol. 2015 Nov;132:190-200. doi: 10.1016/j.jip.2015.10.008. Epub 2015 Oct 28. J Invertebr Pathol. 2015. PMID: 26519623

Impact of fall armyworm (Spodoptera frugiperda) (J.E. Smith) on small-scale maize farmers and its control strategies in the Limpopo province, South Africa.Jamba. 2021 Oct 27;13(1):1016. doi: 10.4102/jamba.v13i1.1016. eCollection 2021. Jamba. 2021. PMID: 34858557 Free PMC article. Review.

Insect prophenoloxidase: the view beyond immunity.Front Physiol. 2014 Jul 11;5:252. doi: 10.3389/fphys.2014.00252. eCollection 2014. Front Physiol. 2014. PMID: 25071597 Free PMC article. Review.

MeSH terms

Substances

LinkOut - more resources

Full Text Sources