The development of artificial diets could considerably simplify and reduce the cost of mass rearing of natural enemies compared to conventional rearing methods. However, improvement of artificial diets can be tedious, convoluted and often uncertain. For accelerating diet development, a better method that can offer informative feedback to target deficiencies in diet improvement is required. Our previous research demonstrated several biological characteristics were diminished in the insect predator, Arma chinensis Fallou, fed on an artificial diet formulated with the aid of transcriptomic methods compared to the Chinese oak silk moth pupae. The present study reports differential proteomic analysis by iTRAQ-PRM, which unravels the molecular mechanism of A. chinensis responding to improvements in the artificial diet. Our study provides multivariate proteomic data and provides comprehensive sequence information in studying A. chinensis. Further, the physiological roles of the differentially expressed proteins and pathways enable us to explain several biological differences between natural prey-fed and improved diet-fed A. chinensis, and subsequent proposed reformulation optimizations to artificial diets.
1
2
3
4
*
Insects
2022,
13(7), 605; https://doi.org/10.3390/insects13070605
Arma chinensisFallou is a predaceous stink bug that can effectively control many kinds of agricultural and forest pests, such as fall armyworm, cotton bollworm and Colorado potato beetle. An insect-free artificial diet comprising chicken egg, tuna fish and raw pig liver was developed for
A. chinensis. Several biological characteristics were diminished for
A. chinensisreared on the artificial diet compared to the pupae of Chinese oak silk moth. Changes in the formulation of the diet were made in response to the transcriptome results and tested using biological characteristics. Several parameters were improved over 6 generations, although the improved artificial diet remained inferior to the pupae of Chinese oak silk moth regarding egg viability, fecundity, body weight, and nymphal development time. The current study reported the differential proteomic analysis revealing the mechanism of
A. chinensisresponding to the improved artificial diet. This information will be used to optimize the formulation of the artificial diet and decrease the cost of mass rearing in
A. chinensis.
Keywords:
Arma chinensis; mass rearing; artificial diet; reformulation; nutriproteomics; biological control
Figure 1
ZIP-Document (ZIP, 3454 KiB)
MDPI and ACS Style
Zou, D.; Coudron, T.A.; Wu, H.; Zhang, L.; Wang, M.; Xu, W.; Xu, J.; Song, L.; Xiao, X. Differential Proteomics Analysis Unraveled Mechanisms of Arma chinensis Responding to Improved Artificial Diet. Insects 2022, 13, 605. https://doi.org/10.3390/insects13070605
AMA Style
Zou D, Coudron TA, Wu H, Zhang L, Wang M, Xu W, Xu J, Song L, Xiao X. Differential Proteomics Analysis Unraveled Mechanisms of Arma chinensis Responding to Improved Artificial Diet. Insects. 2022; 13(7):605. https://doi.org/10.3390/insects13070605
Chicago/Turabian Style
Zou, Deyu, Thomas A. Coudron, Huihui Wu, Lisheng Zhang, Mengqing Wang, Weihong Xu, Jingyang Xu, Liuxiao Song, and Xuezhuang Xiao. 2022. "Differential Proteomics Analysis Unraveled Mechanisms of Arma chinensis Responding to Improved Artificial Diet" Insects 13, no. 7: 605. https://doi.org/10.3390/insects13070605
Find Other Styles