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Title

Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles

Authors

Heba A H Zaghloul; Peter Arensburger; Brian A Federici; Zaghloul; Heba A H; Arensburger; Peter; Federici; Brian A

Availability

Better title

Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles

Source

MDPI (mdpi.com)

URL

https://www.mdpi.com/1999-4915/14/7/1444

Date

2022-06-30

Description

Abstract

Ascoviruses are large DNA viruses that primarily infect lepidopteran larvae. They differ markedly from other plant or animal viruses by initiating replication in the nucleus, then inducing nuclear lysis followed by extensive cellular hypertrophy and subsequent cleavage of the entire enlarged cell into numerous viral vesicles. Most progeny virions are assembled in these vesicles as they circulate in the hemolymph. Here, we report transcriptome studies of host cytoskeletal genes in larvae infected with ascoviruses from 6 h to 21 days post-infection (dpi). We focused on the cabbage looper, Trichoplusia ni, infected with the Trichoplusia ni ascovirus (TnAV), along with supporting studies on the fall armyworm, Spodoptera frugiperda, infected with the Spodoptera frugiperda ascovirus (SfAV). In T. ni, many cytoskeleton genes were upregulated at 48 hours post-infection (hpi), including 29 tubulins, 21 actins, 21 dyneins, and 13 kinesins. Mitochondrial genes were upregulated as much as two-fold at 48 hpi and were expressed at levels comparable to controls in both T. ni and S. frugiperda, even after 21 dpi, when several cytoskeleton genes remained upregulated. Our studies suggest a temporal correlation between increases in the expression of certain host cytoskeletal genes and viral vesicle formation. However, these results need confirmation through functional genetic studies of proteins encoded by these genes.

Keywords

categories = ascovirus,cytoskeleton,mitochondria,RNA-seq,vesicles,<i>Trichoplusia ni</i>,apoptosis,dynein,actin,tubulin

Body

Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles

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Viruses

2022,

14(7), 1444; https://doi.org/10.3390/v14071444

Keywords:ascovirus; cytoskeleton; mitochondria; RNA-seq; vesicles;

Trichoplusia ni; apoptosis; dynein; actin; tubulin

Figure 1

MDPI and ACS Style

Zaghloul, H.A.H.; Arensburger, P.; Federici, B.A. Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles. Viruses 2022, 14, 1444. https://doi.org/10.3390/v14071444

AMA Style

Zaghloul HAH, Arensburger P, Federici BA. Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles. Viruses. 2022; 14(7):1444. https://doi.org/10.3390/v14071444

Chicago/Turabian Style

Zaghloul, Heba A.H., Peter Arensburger, and Brian A. Federici. 2022. "Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles" Viruses 14, no. 7: 1444. https://doi.org/10.3390/v14071444

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