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Title

Investigation of Protein Corona Formed around Biologically Produced Gold Nanoparticles

Authors

Parastoo Pourali; Eva Neuhöferová; Volha Dzmitruk; Veronika Benson; Pourali; Parastoo; Neuhöferová; Eva; Dzmitruk; Volha; Benson; Veronika

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Better title

Investigation of Protein Corona Formed around Biologically Produced Gold Nanoparticles

Source

MDPI (mdpi.com)

URL

https://www.mdpi.com/1996-1944/15/13/4615

Date

2022-06-30

Description

Abstract

Although there are several research articles on the detection and characterization of protein corona on the surface of various nanoparticles, there are no detailed studies on the formation, detection, and characterization of protein corona on the surface of biologically produced gold nanoparticles (AuNPs). AuNPs were prepared from Fusarium oxysporum at two different temperatures and characterized by spectrophotometry, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDS). The zeta potential of AuNPs was determined using a Zetasizer. AuNPs were incubated with 3 different concentrations of mouse plasma, and the hard protein corona was detected first by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then by electrospray liquid chromatography–mass spectrometry (LC-MS). The profiles were compared to AuNPs alone that served as control. The results showed that round and oval AuNPs with sizes below 50 nm were produced at both temperatures. The AuNPs were stable after the formation of the protein corona and had sizes larger than 86 nm, and their zeta potential remained negative. We found that capping agents in the control samples contained small peptides/amino acids but almost no protein(s). After hard protein corona formation, we identified plasma proteins present on the surface of AuNPs. The identified plasma proteins may contribute to the AuNPs being shielded from phagocytizing immune cells, which makes the AuNPs a promising candidate for in vivo drug delivery. The protein corona on the surface of biologically produced AuNPs differed depending on the capping agents of the individual AuNP samples and the plasma concentration.

Keywords

categories = biologically produced gold nanoparticles,hard protein corona,capping agent,<i>Fusarium oxysporum</i>

Body

Investigation of Protein Corona Formed around Biologically Produced Gold Nanoparticles

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Materials

2022,

15(13), 4615; https://doi.org/10.3390/ma15134615

Keywords:biologically produced gold nanoparticles; hard protein corona; capping agent;

Fusarium oxysporum

Figure 1

MDPI and ACS Style

Pourali, P.; Neuhöferová, E.; Dzmitruk, V.; Benson, V. Investigation of Protein Corona Formed around Biologically Produced Gold Nanoparticles. Materials 2022, 15, 4615. https://doi.org/10.3390/ma15134615

AMA Style

Pourali P, Neuhöferová E, Dzmitruk V, Benson V. Investigation of Protein Corona Formed around Biologically Produced Gold Nanoparticles. Materials. 2022; 15(13):4615. https://doi.org/10.3390/ma15134615

Chicago/Turabian Style

Pourali, Parastoo, Eva Neuhöferová, Volha Dzmitruk, and Veronika Benson. 2022. "Investigation of Protein Corona Formed around Biologically Produced Gold Nanoparticles" Materials 15, no. 13: 4615. https://doi.org/10.3390/ma15134615

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