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Title

Assessment of the Potential of a Native Non-Aflatoxigenic Aspergillus flavus Isolate to Reduce Aflatoxin Contamination in Dairy Feed

Authors

Erika Janet Rangel-Muñoz; Arturo Gerardo Valdivia-Flores; Sanjuana Hernández-Delgado; Carlos Cruz-Vázquez; María Carolina de-Luna-López; Teódulo Quezada-Tristán; Raúl Ortiz-Martínez; Netzahualcóyotl Mayek-Pérez; Rangel-Muñoz; Erika Janet; Valdivia-Flores; Arturo Gerardo; Hernández-Delgado; Sanjuana; Cruz-Vázquez; Carlos; De-Luna-López; María Carolina; Quezada-Tristán; Teódulo; Ortiz-Martínez; Raúl; Mayek-Pérez; Netzahualcóyotl

Availability

Better title

Assessment of the Potential of a Native Non-Aflatoxigenic Aspergillus flavus Isolate to Reduce Aflatoxin Contamination in Dairy Feed

Source

MDPI (mdpi.com)

URL

https://www.mdpi.com/2072-6651/14/7/437

Date

2022-06-27

Description

Abstract

Aspergillus species can produce aflatoxins (AFs), which can severely affect human and animal health. The objective was to evaluate the efficacy of reducing AF contamination of a non-aflatoxigenic isolate of A. flavus experimentally coinoculated with different aflatoxigenic strains in whole plant (WP), corn silage (CS), immature grains (IG) and in culture media (CM). An L-morphotype of A. flavus (CS1) was obtained from CS in a dairy farm located in the Mexican Highland Plateau; The CS1 failed to amplify the AFs biosynthetic pathway regulatory gene (aflR). Monosporic CS1 isolates were coinoculated in WP, CS, IG and CM, together with A. flavus strains with known aflatoxigenic capacity (originating from Cuautitlán and Tamaulipas, Mexico), and native isolates from concentrate feed (CF1, CF2 and CF3) and CS (CS2, CS3). AF production was evaluated by HPLC and fungal growth rate was measured on culture media. The positive control strains and those isolated from CF produced a large average amount of AFs (15,622 ± 3952 and 12,189 ± 3311 µg/kg), whereas A. flavus strains obtained from CS produced a lower AF concentration (126 ± 25.9 µg/kg). CS1 was efficient (p < 0.01) in decreasing AF concentrations when coinoculated together with CF, CS and aflatoxigenic positive control strains (71.6–88.7, 51.0–51.1 and 63.1–71.5%) on WP, CS, IG and CM substrates (73.9–78.2, 65.1–73.7, 63.8–68.4 and 57.4–67.6%). The results suggest that the non-aflatoxigenic isolate can be an effective tool to reduce AF contamination in feed and to minimize the presence of its metabolites in raw milk and dairy products intended for human nutrition.

Keywords

categories = mycotoxins,biological control,aflatoxin biocontrol agents,Mexico,dairy cows

Body

Assessment of the Potential of a Native Non-Aflatoxigenic Aspergillus flavus Isolate to Reduce Aflatoxin Contamination in Dairy Feed

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Toxins

2022,

14(7), 437; https://doi.org/10.3390/toxins14070437

Keywords:mycotoxins; biological control; aflatoxin biocontrol agents; Mexico; dairy cows

Figure 1

MDPI and ACS Style

Rangel-Muñoz, E.J.; Valdivia-Flores, A.G.; Hernández-Delgado, S.; Cruz-Vázquez, C.; de-Luna-López, M.C.; Quezada-Tristán, T.; Ortiz-Martínez, R.; Mayek-Pérez, N. Assessment of the Potential of a Native Non-Aflatoxigenic Aspergillus flavus Isolate to Reduce Aflatoxin Contamination in Dairy Feed. Toxins 2022, 14, 437. https://doi.org/10.3390/toxins14070437

AMA Style

Rangel-Muñoz EJ, Valdivia-Flores AG, Hernández-Delgado S, Cruz-Vázquez C, de-Luna-López MC, Quezada-Tristán T, Ortiz-Martínez R, Mayek-Pérez N. Assessment of the Potential of a Native Non-Aflatoxigenic Aspergillus flavus Isolate to Reduce Aflatoxin Contamination in Dairy Feed. Toxins. 2022; 14(7):437. https://doi.org/10.3390/toxins14070437

Chicago/Turabian Style

Rangel-Muñoz, Erika J., Arturo G. Valdivia-Flores, Sanjuana Hernández-Delgado, Carlos Cruz-Vázquez, María C. de-Luna-López, Teódulo Quezada-Tristán, Raúl Ortiz-Martínez, and Netzahualcóyotl Mayek-Pérez. 2022. "Assessment of the Potential of a Native Non-Aflatoxigenic Aspergillus flavus Isolate to Reduce Aflatoxin Contamination in Dairy Feed" Toxins 14, no. 7: 437. https://doi.org/10.3390/toxins14070437

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