A protein fragment ( 941 1343 ) from mutant __NODE__ protein ( Y1316F Y1322F with its C terminal domain mutated Y960F with its juxtamembrane domain mutated and with its kinase domain retained ) increases phosphorylation of a protein fragment ( 516 777 ) from __SP__ __NODE__ protein to a phosphorylated ( S ) protein fragment ( 516 777 ) from __SP__ __NODE__ protein .