Binding of a DNA fragment containing a __NODE__ __NODE__ response element and a protein fragment ( 613 752 ) containing a LXXLL motif 1 ( aa sequence LLQLL ) and a LXXLL motif 2 ( aa sequence LHKLL ) and a LXXLL motif 3 ( aa sequence LRYLL ) and a receptor binding domain from __NODE__ protein and a protein protein complex consisting of mutant __NODE__ ( C terminal truncation 403 End with its AF 2 transcription activation domain deleted ) and of __NODE__ does not occur in a cell free system .