Binding of a protein chimera composed of a protein fragment containing a __NODE__ rich domain 1 and a __NODE__ rich domain 3 and a N terminal domain from __SP__ __NODE__ protein and of a protein fragment containing a __NODE__ rich domain 2 from __SP__ __NODE__ protein and of a __NODE__ Fc region protein fragment and a protein fragment ( 103 281 ) from __SP__ __NODE__ protein does not occur in a cell free system .