Binding of __SP__ __NODE__ protein and a protein DNA complex consisting of promoter fragment ( 5 ' AGCTGGACCAGGACAAAGGTCACGTTCAGCT 3 ' containing a __NODE__ response element from __NODE__ gene(s) and of mutant __SP__ __NODE__ ( deletion 12H ) and of mutant __SP__ __NODE__ ( deletion 12H ) occurs in a cell free system .