Cyclic __NODE__ in culture medium is not necessary for binding of a DNA fragment ( 5 ' TCGACTCCCGAACGTGACCTTTGTCCTGGTCCCCTGTCGAC 3 ' containing a DR1 from __NODE__ gene in __SP__ cell extract and heterodimer consisting of __NODE__ and of __NODE__ in __SP__ cell extract and mutant __NODE__ protein ( N terminal truncation 1 2238 ) in __SP__ cell extract .