In 130.5 day old embryonic __SP__ , mutant __SP__ __NODE__ gene ( homozygous knockout ) and mutant __SP__ __NODE__ gene ( homozygous knockout ) cause little or no change in release of __SP__ __NODE__ protein from mitochondria from 130.5 day old embryonic __SP__ fibroblasts treated with __NODE__ protein to cytoplasm from 130.5 day old embryonic __SP__ fibroblasts treated with anti __NODE__ protein antibody .