In 3T3 L1 cells , a protein chimera composed of a protein fragment ( 1 212 ) containing a transcription activation domain from __NODE__ protein and of a protein fragment ( 274 359 ) containing a dimerization domain and a DNA binding domain from __NODE__ protein in 3T3 L1 cells causes little or no change in trans activation of a luciferase reporter gene with a DNA endogenous promoter from the __SP__ __NODE__ gene .