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Ingenuity178

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Phrase 4 - PMID ?

In a cell free system , binding of __SP__ __NODE__ protein and immobilized purified mutant __SP__ __NODE__ protein ( deletion 112 1509 with its extracytoplasmic repeat 2 10 deleted and with its extracytoplasmic repeat 1 mutated and with its signal peptide retained C terminal truncation 1652 End with its extracytoplasmic repeat 12 15 deleted ) from conditioned medium Cos 7 cells is greater than binding of immobilized purified mutant __SP__ __NODE__ protein ( deletion 112 1509 with its extracytoplasmic repeat 2 10 deleted and with its extracytoplasmic repeat 1 mutated and with its signal peptide retained C terminal truncation 1652 End with its extracytoplasmic repeat 12 15 deleted ) from conditioned medium Cos 7 cells and __NODE__ protein .


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                                                                                                                                                                                                                                                                                                                                                   +--------------OBJ:V-N-------------+                                                                                                                                                                                                                                       +-------------------------COMP:V-N(with)-------------------------+                                                                             
                                                                                                                          +------------------OBJ:V-N-----------------+   +---------------------SUBJ:V_PASS-N--------------------+                                               +-------------------COMP:ADJ-N(with)------------------+            +---------COMP:V-N(from)--------+  |                                                                                                                                                                                                                                       +-------------OBJ:V-N------------+                               |                                                                             
             +-------------OBJ:V-N-------------+       +--------------------SUBJ:V-N-------------------+                  |          +------------SUBJ:V-N-----------+   +OBJ:V-N+                                              |                                               |                          +------------OBJ:V-N------------+       |                +-MOD_ATT:N-ADJ+  |   +--------------OBJ:V-N--------------+                                                          +--------------SUBJ:V-N--------------+      +------------------OBJ:V-N-----------------+                                             |    +--MOD_ATT:N-N--+           |    +------------OBJ:V-N------------+OBJ:V-N+---------COMP:V-N(from)--------+                                
             |              +--OBJ:V-N-+       |       |                                       +SUBJ:V-+------OBJ:V-N-----+          +---COMP:N-N(with)--+           |   |  +SUBJ+                         +MOD_ATT:N-A+SUBJ:V_P+----COMP:V_PASS-N(with)----+SUBJ:V_P+          |                          |              +MOD_ATT:N-A+    |  +SUBJ+                |         +MOD_+  |   |         +MOD_ATT:N-AD+--SUBJ:V-N--+                  +-MOD_ATT:N-ADJ+                        +-----COMP:N-N(with)-----+           +OBJ:V-+                              +--SUBJ:V-N-+   +SUBJ+                    +MOD_AT+SUBJ:V-N+    |     +MOD_ATT:N+MOD_ATT:N-N+    |              +MOD_ATT:N-A+    |  +SUBJ+                +MOD_ATT:N+MOD_+  +MOD+--SUBJ:V-N-+OBJ:V-N+     
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 In a cell free system , binding of __SP__ __NODE__ protein and immobilized purified mutant __SP__ __NODE__ protein ( deletion 112 1509 with its extracytoplasmic repeat 2 10 deleted and with its extracytoplasmic repeat 1 mutated and with its signal peptide retained C terminal truncation 1652 End with its extracytoplasmic repeat 12 15 deleted ) from conditioned medium Cos 7 cells is greater than binding of immobilized purified mutant __SP__ __NODE__ protein ( deletion 112 1509 with its extracytoplasmic repeat 2 10 deleted and with its extracytoplasmic repeat 1 mutated and with its signal peptide retained C terminal truncation 1652 End with its extracytoplasmic repeat 12 15 deleted ) from conditioned medium Cos 7 cells and __NODE__ protein .
OBJ:V-N (free,__NODE__)
OBJ:V-N (bind,__SP__)
SUBJ:V-N (__NODE__,protein)
SUBJ:V-N (__NODE__,__SP__)
OBJ:V-N (__NODE__,deletion)
COMP:N-N(with) (@card@,extracytoplasmic)
OBJ:V-N (repeat,deletion)
SUBJ:V-N (repeat,@card@)
OBJ:V-N (delete,2)
SUBJ:V-N (delete,@card@)
MOD_ATT:N-ADJ (repeat,extracytoplasmic)
SUBJ:V_PASS-N (mutate,2)
SUBJ:V_PASS-N (mutate,repeat)
COMP:V_PASS-N(with) (mutate,peptide)
SUBJ:V_PASS-N (retain,peptide)
COMP:ADJ-N(with) (terminal,repeat)
OBJ:V-N (with,@card@)
MOD_ATT:N-ADJ (repeat,extracytoplasmic)
SUBJ:V-N (delete,@card@)
COMP:V-N(from) (delete,Cos)
OBJ:V-N (delete,7)
MOD_ATT:N-ADJ (Cos,condition)
MOD_ATT:N-ADJ (Cos,medium)
MOD_ATT:N-ADJ (bind,great)
OBJ:V-N (immobilize,cell)
SUBJ:V-N (immobilize,bind)
MOD_ATT:N-ADJ (__NODE__,mutant)
COMP:N-N(with) (@card@,extracytoplasmic)
SUBJ:V-N (repeat,@card@)
OBJ:V-N (repeat,@card@)
OBJ:V-N (repeat,@card@)
SUBJ:V-N (repeat,extracytoplasmic)
SUBJ:V_PASS-N (mutate,1)
MOD_ATT:N-N (peptide,signal)
SUBJ:V-N (retain,peptide)
OBJ:V-N (retain,end)
COMP:V-N(with) (retain,repeat)
MOD_ATT:N-N (truncation,C)
MOD_ATT:N-ADJ (truncation,terminal)
MOD_ATT:N-N (end,truncation)
OBJ:V-N (with,@card@)
MOD_ATT:N-ADJ (repeat,extracytoplasmic)
OBJ:V-N (delete,@card@)
SUBJ:V-N (delete,@card@)
COMP:V-N(from) (delete,Cos)
MOD_ATT:N-ADJ (medium,condition)
MOD_ATT:N-N (Cos,medium)
MOD_ATT:N-ADJ (cell,7)
SUBJ:V-N (__NODE__,cell)
OBJ:V-N (__NODE__,protein)