In HEK293 cells , binding of mutant __NODE__ protein ( Y18F with its phosphorylation site mutated ) and Sh2 b beta [__NODE__] protein is greater than binding of mutant __NODE__ protein ( Y18F with its phosphorylation site mutated ) and mutant Sh2 b beta [__NODE__] protein ( N terminal truncation 1 397 with its pleckstrin homology ( PH ) domain deleted and with its SH2 domain retained ) .