In Hela cells , a protein chimera composed of a protein fragment ( 1 147 ) containing a DNA binding domain from __SP__ Gal4 protein and of a protein fragment ( 185 438 ) containing a ligand binding domain from __SP__ __NODE__ protein and WY 14643 and __SP__ __NODE__ protein increase activation of a __NODE__ reporter gene with a synthetic promoter that has a Gal4 UAS response element .