In lysate from Cos 7 cells , binding of a DNA fragment ( AGCTGCACTGGGTGAATGAGGACATTACA ) containing a VDRE from __SP__ __NODE__ gene and mutant __SP__ __NODE__ protein ( I314S ) and __NODE__ protein is greater than binding of a DNA fragment ( AGCTGCACTGGGTGAATGAGGACATTACA ) containing a VDRE from __SP__ __NODE__ gene and mutant __SP__ __NODE__ protein ( I314S ) and mutant __NODE__ protein ( R391C ) .