In reticulocyte lysates , binding of mutant __SP__ __NODE__ protein ( N terminal truncation 1 205 with its DBD protein protein interaction domain deleted and with its ligand binding domain retained ) and __SP__ __NODE__ protein is greater than binding of mutant __SP__ __NODE__ protein ( C terminal truncation 240 End with its ligand binding domain mutated and with its DBD protein protein interaction domain retained ) and __SP__ __NODE__ protein .