FastQCFastQC Report
ven. 24 févr. 2017
SRR1145846_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1145846_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52621
Sequences flagged as poor quality0
Sequence length47-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATACAAATTGCAGACTGCAGCGTTCTGAGAAACATCTTTGTGATGTT3800.7221451511753862No Hit
GGAAAGGAAACGAGTGGAATGGAATGGAGTGGAATGGCTTGAAATGGATA3220.611922996522301No Hit
TCTTCTTGTTCTTCTCCAGCGGTTTGGGTACCAACGTCTTCGGTAGAAGT2600.49409931396210643No Hit
ATTCATACAAATTGCAGACTGCAGCGTTCTGAGAAACATCTTTGTGATGT1110.21094239942228388No Hit
GGGAATTCATACAAATTGCAGACTGCAGCGTTCTGAGAAACATCTTTGTG1010.19193857965451055No Hit
CTCTGTGTCCTGAATACAAACATCACAAAGATGTTTCTCAGAACGCTGCA990.1881378157009559No Hit
TGAGGATTTCGTTGGAAGCGGGAATTCATACAAATTGCAGACTGCAGCGT940.17863590581706923No Hit
GAAAAAATAAAAACCGTAGATTAACTAGCAAAGAAAGAGCAGATGACTTA900.1710343779099599No Hit
TCATACAAATTGCAGACTGCAGCGTTCTGAGAAACATCTTTGTGATGTTT810.1539309401189639No Hit
GAGGATTTCGTTGGAAGCGGGAATTCATACAAATTGCAGACTGCAGCGTT680.1292259744208586No Hit
TTGATTCTCTGTTAGGGGTTTTCTATACTTGTCTACTTGAGGTTTTCTAT670.12732559244408126No Hit
GAATTCATACAAATTGCAGACTGCAGCGTTCTGAGAAACATCTTTGTGAT660.12542521046730393No Hit
GGAATTCATACAAATTGCAGACTGCAGCGTTCTGAGAAACATCTTTGTGA650.12352482849052661No Hit
CTTTCTGCAAAGTATTTATCTAGTGGTACGTATGCTGTTCTAGGTGGGTA620.1178236825601946No Hit
TTCGACGAAGGAGACTTAGACCGCCTTTTCGAAGAAGATTTCACAGAAGA600.11402291860663992No Hit
GGATTTCGTTGGAAGCGGGAATTCATACAAATTGCAGACTGCAGCGTTCT580.11022215465308527No Hit
CAAATTGCAGACTGCAGCGTTCTGAGAAACATCTTTGTGATGTTTGTATT580.11022215465308527No Hit
AAAATATATTATTTTTTAGGTATGAGCAGCTACCTAACTCCAACAATATA540.10262062674597594No Hit
GGGTGAGTCTGACAGGTTGCTTCTAGGGGGTCTGTTAAAAGCTCTGCAGG540.10262062674597594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATCG203.001951E-6365.97766145
AGGGTCC250.0028864841195.18808145
GTAGGCC300.004980264162.65672145
GCGGGTT100.0077735446140.483324
AGGGCGA100.0077735446140.483325
GGGGGTA100.0077735446140.483324
TGGCGGG301.8815606E-593.3884661
GGGGGGT502.7921487E-884.209713
CTTGTTC555.3716576E-876.6272665
AAACGAG651.9608706E-975.644868
AACGAGT703.5252015E-970.241669
GCGGGGG407.762011E-570.174773
TCTTGTT651.7084312E-764.8384554
CTTCTTG451.4036863E-462.2589762
TGGGGGG350.00406725760.0354421
GAAACGA1051.4224497E-953.5174527
GTTCTTC553.7303934E-451.0848438
GGGGGTG553.7303934E-451.0848434
TTCTTGT851.0971489E-649.5351263
GGCGGGG605.811167E-446.6942332
Produced by FastQC (version 0.11.3)