Binding of promoter fragment containing a __NODE__ binding site ( 5 ' TGTTTCA 3 ' and a __NODE__ response element ( 5 ' GGAAAA 3 ' from mutant __SP__ __NODE__ gene ( T?C G?T T?G T?G T?A C?A A?T with its __NODE__ binding site mutated ) and __NODE__ protein does not occur in a cell free system .