In a system of purified components , binding of promoter fragment from __SP__ __NODE__ gene consisting of __NODE__ response element and a protein fragment ( 1 257 ) containing a winged helix domain from __NODE__ protein is greater than binding of promoter fragment from __SP__ __NODE__ gene consisting of __NODE__ response element and a mutant protein fragment ( 1 257 ) ( H215R ) containing a winged helix domain from __NODE__ protein .