In a system of purified components , binding of __SP__ __NODE__ protein and __SP__ __NODE__ protein and __SP__ __NODE__ protein containing a carboxy terminal domain and a linker domain and a N terminal domain is greater than binding of mutant __SP__ __NODE__ protein ( Y27L ) and __SP__ __NODE__ protein and __SP__ __NODE__ protein containing a carboxy terminal domain and a linker domain and a N terminal domain .