In DU145 cells , binding of a protein fragment ( 1 401 ) from __NODE__ protein and a protein fragment containing a ligand binding domain from __SP__ __NODE__ protein is greater than binding of a mutant protein fragment ( 1 401 ) ( L95A L96A with its LXXLL motif mutated ) from __NODE__ protein and a protein fragment containing a ligand binding domain from __SP__ __NODE__ protein .