In DU145 cells , the degree of binding of a protein fragment ( 1 401 ) containing a Fxxlf motif and a LXXLL motif from __NODE__ protein and a protein fragment containing a ligand binding domain from __NODE__ protein is greater than the degree of binding of a protein fragment ( 1 401 ) containing a Fxxlf motif from mutant __NODE__ protein ( L95A L96A with its LXXLL motif mutated ) and a protein fragment containing a ligand binding domain from __NODE__ protein .