In Hep3B cells , __NODE__ binding site 1 ( 5 ' GTTAATCAGAAAA 3 ' from __SP__ __NODE__ gene and __NODE__ binding site 2 ( 5 ' CTTACTCAATAA 3 ' from __SP__ __NODE__ gene are necessary for maximal trans activation of reporter gene with a DNA endogenous promoter from the __SP__ __NODE__ gene containing a __NODE__ binding site 1 2 that is increased by __NODE__ protein .