In HepG2 cells , a transcriptionally active protein fragment ( 1 468 ) containing a N terminal domain from __SP__ __NODE__ protein increases activation of promoter fragment containing a repeated ( 2 times ) __NODE__ binding site and a __NODE__ response element 1 from __NODE__ gene that is increased by constitutively active mutant __NODE__ protein ( deletion 32 51 S218E S222E ) .