In lysate from Hela cells , binding of a protein chimera composed of __NODE__ protein and of a protein fragment containing a __NODE__ binding domain from __SP__ viral E7 protein and __SP__ __NODE__ protein is the same as binding of a protein chimera composed of mutant __NODE__ protein ( R285E K365E R474E R521E R524E ) and of a protein fragment containing a __NODE__ binding domain from __SP__ viral E7 protein and __SP__ __NODE__ protein .