__NODE__ protein ( with a treatment period of 24 hours ) causes little or no change in content of __NODE__ in cytosol from fetal interscapular __SP__ brown adipose tissue serum deprived immortalized brown adipocytes that is decreased by mutant __SP__ __NODE__ gene ( homozygous knockout ) in serum deprived immortalized brown adipocytes from fetal interscapular __SP__ brown adipose tissue .