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Ingenuity433


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Phrase 10 - PMID ?

The affinity of binding of __SP__ __NODE__ protein in a cell fraction from Cho cells and __SP__ __NODE__ protein in a cell fraction from Cho cells is greater than or equal to the affinity of binding of __SP__ __NODE__ protein in a system of purified components and monomeric soluble mutant __SP__ __NODE__ A protein ( deletion 474 703 with its __NODE__ type III domain 1 2 deleted and with its carboxy terminal domain and its CR domain and its linker domain 1 2 ( aa sequence __NODE__ ) and its loop domain 1 2 retained ) in a system of purified components .


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                                                                                                     +----------------------------------COMP:V-N(from)----------------------------------+                                                                                                                                                                                                                                                                                                                                                                                      
                                                                                                     |                                          +---------------------------------COMP:N-N(of)--------------------------------+                                 +-------------------SUBJ:V-N------------------+                          +-----------COMP:N-N(with)-----------+                                                                                              +--------------SUBJ:V-N--------------+                                                            
                    +--------MOD_ATT:N-N-------+                  +-------------SUBJ:V-N-------------+--------------COMP:V-N(from)--------------+                                       +-------------COMP:N-N(of)------------+                                 |             +-----MOD_ATT:N-ADJ-----+       |                          |                   +--MOD_ATT:N-ADJ-+-----------------COMP:N-N(with)-----------------+            +--------MOD_ATT:N-N-------+     +------SUBJ:V-N-----+                |                                                            
                    |          +-MOD_ATT:N-ADJ-+                  +-COMP:N-N(from)+                  +-------COMP:V-N(in)-------+               |                                       |          +-------MOD_ATT:N-ADJ------+--------OBJ:V-N--------+         |             |        +-MOD_ATT:N-ADJ+       +----------OBJ:V-N---------+                   |      +MOD_ATT:N+                               +--MOD_ATT:N-ADJ-+            |    +-----MOD_ATT:N-N-----+     +---APPOS--+        +----------OBJ:V-N---------+OBJ:V-N+              +----COMP:N-N(of)----+      
                    |          |       +MOD_ATT+                  |          +MOD_+          +SUBJ:V-+                   +MOD_AT+          +MOD_+                                       |          |          +-MOD_ATT:N-ADJ-+            +-SUBJ:V-N-+         |             |        |       +MOD_AT+SUBJ:V-+    +MOD_+---APPOS--+     |                   |      |   +MOD_A+                               |        +MOD_ATT+            |    |              +MOD_AT+     |    +MOD_A+        |                +-OBJ:V-N-+ +SUBJ:+-COMP:V-N(in)-+          +MOD_ATT:N+      
                    |          |       |       |                  |          |    |          |       |                   |      |          |    |                                       |          |          |               |            |          |         |             |        |       |      |       |    |    |          |     |                   |      |   |     |                               |        |       |            |    |              |      |     |    |     |        |                |         | |     |              |          |         |      
 The affinity of binding of __SP__ __NODE__ protein in a cell fraction from Cho cells and __SP__ __NODE__ protein in a cell fraction from Cho cells is greater than or equal to the affinity of binding of __SP__ __NODE__ protein in a system of purified components and monomeric soluble mutant __SP__ __NODE__ A protein ( deletion 474 703 with its __NODE__ type III domain 1 2 deleted and with its carboxy terminal domain and its CR domain and its linker domain 1 2 ( aa sequence __NODE__ ) and its loop domain 1 2 retained ) in a system of purified components .
MOD_ATT:N-N (protein,bind)
MOD_ATT:N-ADJ (protein,__SP__)
MOD_ATT:N-ADJ (protein,__NODE__)
COMP:N-N(from) (fraction,cell)
MOD_ATT:N-N (cell,Cho)
SUBJ:V-N (__NODE__,fraction)
SUBJ:V-N (__NODE__,__SP__)
COMP:V-N(in) (__NODE__,fraction)
COMP:V-N(from) (__NODE__,cell)
COMP:V-N(from) (__NODE__,affinity)
MOD_ATT:N-N (fraction,cell)
MOD_ATT:N-N (cell,Cho)
COMP:N-N(of) (cell,protein)
COMP:N-N(of) (affinity,protein)
MOD_ATT:N-ADJ (protein,bind)
MOD_ATT:N-ADJ (protein,__SP__)
OBJ:V-N (purify,protein)
SUBJ:V-N (purify,system)
MOD_ATT:N-ADJ (__SP__,monomeric)
MOD_ATT:N-ADJ (__SP__,soluble)
MOD_ATT:N-ADJ (__SP__,mutant)
SUBJ:V-N (__NODE__,component)
SUBJ:V-N (__NODE__,__SP__)
OBJ:V-N (__NODE__,@card@)
MOD_ATT:N-N (protein,a)
APPOS (protein,deletion)
COMP:N-N(with) (@card@,domain)
MOD_ATT:N-ADJ (domain,__NODE__)
MOD_ATT:N-N (domain,type)
MOD_ATT:N-N (domain,III)
COMP:N-N(with) (domain,domain)
MOD_ATT:N-ADJ (domain,carboxy)
MOD_ATT:N-ADJ (domain,terminal)
MOD_ATT:N-N (domain,CR)
MOD_ATT:N-N (domain,domain)
MOD_ATT:N-N (domain,linker)
APPOS (2,sequence)
MOD_ATT:N-N (sequence,aa)
SUBJ:V-N (__NODE__,2)
OBJ:V-N (__NODE__,1)
SUBJ:V-N (loop,2)
OBJ:V-N (loop,1)
OBJ:V-N (retain,1)
SUBJ:V-N (retain,2)
COMP:V-N(in) (retain,system)
COMP:N-N(of) (system,component)
MOD_ATT:N-ADJ (component,purify)