The affinity of binding of __NODE__ protein in a system of purified components and __NODE__ protein in a cell free system and __SP__ __NODE__ protein in a system of purified components is the same as the affinity of binding of __NODE__ protein in a system of purified components and __NODE__ protein in a cell free system and mutant __SP__ __NODE__ protein ( K228M G229D R230G K231E R232A ) in a system of purified components .