25 hydroxycholesterol and __NODE__ increase polyubiquitination of mutant __NODE__ protein ( C terminal truncation 351 End with its __NODE__ sensing domain and its transmembrane domain 1 8 retained ) to polyubiquitinated ( K248 ) mutant __NODE__ protein ( C terminal truncation 351 End with its __NODE__ sensing domain and its transmembrane domain 1 8 retained ) .