Binding of promoter fragment ( AGGCCGAGTTTTTTTTTTGCGCTCG 240 216 ) from mutant __SP__ __NODE__ gene ( A 232T C 230T A 229T C 228T C 227T C 225T A 224T G 223T with its __NODE__ regulatory element mutated ) and __SP__ __NODE__ protein does not occur in a system of purified components .