Binding of promoter fragment ( CATTCTGGGTCAAAGTTGATCCCCT ) from mutant __NODE__ gene(s) ( G 340A G 338A T 337A ) consisting of DR1 response element and a protein protein complex consisting of frog __NODE__ and of mutant __SP__ __NODE__ ( E?G G?S G?V with its P box mutated ) does not occur in a cell free system .