Binding of promoter fragment ( CATTCTGGGTCAAAGTTGATCCCCT ) from mutant __NODE__ gene(s) ( T 331A T 330A G 329C ) consisting of DR1 response element and a protein protein complex consisting of mutant frog __NODE__ ( E?G G?S G?V with its P box mutated ) and of __SP__ __NODE__ does not occur in a cell free system .