In a system of purified components , the affinity of binding of mutant promoter fragment ( 590 581 ) ( T 582A ) containing a SRE from __NODE__ c [__NODE__] gene and SREBP 1C protein is greater than the affinity of binding of promoter fragment ( 590 581 ) containing a SRE from __NODE__ c [__NODE__] gene and SREBP 1C protein .