In primary vascular smooth muscle cells from male adult __SP__ thoracic aorta , __NODE__ protein and __NODE__ protein cause little or no change in activity of a luciferase reporter gene with a DNA endogenous promoter ( 1153 46 ) from the mutant __SP__ __NODE__ gene ( A 922G A 921C C 916A C 915G with its PPRE mutated ) that is increased by __NODE__ protein .