In JEG3 cells , a transcriptionally active protein fragment ( 1 460 ) from __SP__ __NODE__ protein causes little or no change in activation of reporter gene with a DNA endogenous promoter ( 334 314 ) from the mutant __SP__ __NODE__ gene ( A 232T C 230T A 229T C 228T C 227T C 225T A 224T G 223T with its __NODE__ regulatory element mutated ) that has a __NODE__ response element and a GC box .