In JEG3 cells , a transcriptionally active protein fragment ( 1 460 ) from __SP__ __NODE__ protein causes little or no change in activation of reporter gene with a DNA endogenous promoter ( 334 314 ) from the mutant __SP__ __NODE__ gene ( T 251A G 250A A 249T C 248G G 245C with its __NODE__ response element mutated ) that has a GC box and a __NODE__ regulatory element .