In JEG3 cells , a transcriptionally active protein fragment ( 1 460 ) from __SP__ __NODE__ protein increases activation of reporter gene with a DNA endogenous promoter ( 334 314 ) from the mutant __SP__ __NODE__ gene ( G 277T G 276T C 275T G 274T with its GC box mutated ) that has a __NODE__ response element and a __NODE__ regulatory element .