In nuclear extract from 220.0 day old fetal __SP__ parietal bone primary osteoblasts , binding of promoter fragment ( 79 43 ) containing a C/__NODE__ binding site and a E box motif from __SP__ __NODE__ gene and protein is greater than binding of promoter fragment ( 79 43 ) containing a E box motif from mutant __SP__ __NODE__ gene ( T 65C T 64G G 63C with its C/__NODE__ binding site mutated ) and protein .