In nuclear extract from Cos 7 cells , the affinity of binding of promoter fragment ( 291 268 ) containing a __NODE__ binding site 1 ( 5 ' GTTAATCAGAAAA 3 ' from __SP__ __NODE__ gene and __NODE__ protein is greater than the affinity of binding of promoter fragment ( 125 102 ) containing a C/__NODE__ binding site and a __NODE__ binding site 2 ( 5 ' CTTACTCAATAA 3 ' from __SP__ __NODE__ gene and __NODE__ protein .