In nuclear extract from Cos cells , the affinity of binding of promoter fragment containing a mutant __NODE__ binding site 2 ( G?C A?T A?T ) from __NODE__ gene and __NODE__ protein is the same as the affinity of binding of promoter fragment containing a mutant __NODE__ binding site 2 ( G?C A?T A?T ) from __NODE__ gene and mutant __NODE__ protein ( R474A N475A T541G with its __NODE__ homology domain mutated ) .