In nuclear extract from H 411E cells , binding of a DNA fragment ( 433 396 ) containing a AF1 and a AF 2 binding site and a __NODE__ binding site 1 2 from __NODE__ gene and __SP__ __NODE__ protein(s) is the same as binding of a mutant DNA fragment ( 433 396 ) ( T 409G T 408G with its AF 2 binding site mutated ) containing a AF1 and a __NODE__ binding site 1 2 from __NODE__ gene and __SP__ __NODE__ protein(s) .