In nuclear extract from NIH/3T3 cells , binding of promoter fragment ( 946 934 ) ( 5 ' AGCTTCTTTTCAAGATTG 3 ' containing a C/__NODE__ binding site from __SP__ __NODE__ gene and protein is greater than binding of mutant promoter fragment ( 946 934 ) ( insertion 942 941T ) ( 5 ' AGCTTCTTTTCTAAGATTG 3 ' containing a C/__NODE__ binding site from __SP__ __NODE__ gene and protein .