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In a system of purified components , the affinity of binding of __SP__ __NODE__ protein and a protein protein complex consisting of cleaved __NODE__ containing a catalytic domain and of cleaved __NODE__ containing a pro domain is the same as the affinity of binding of __SP__ __NODE__ protein and a protein protein complex consisting of cleaved __NODE__ containing a pro domain and of mutant cleaved __NODE__ ( S386A ) lacking catalytic activity and containing a catalytic domain .


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                                                                                                                                                                                                                                                                                                                                                                                                                     +----------------APPOS---------------+                                       
                                                         +----------OBJ:V-N---------+                                                   +----------------------------OBJ:V-N---------------------------+                                                                                                                                                      +---------------------COMP:N-N(of)---------------------+        +--------MOD_ATT:N-N--------+                                       
                                                         |          +-MOD_ATT:N-ADJ-+                                                   |                  +-------OBJ:V-N------+                      |         +----OBJ:V-N---+                                                +---------------------MOD_ATT:N-ADJ---------------------+                    |         +----OBJ:V-N---+             +-MOD_ATT:N-ADJ-+        |        +----MOD_ATT:N-N---+             +-------OBJ:V-N------+    
         +-SUBJ:V-N-+-OBJ:V-N-+                          |          |       +MOD_ATT+             +---------------SUBJ:V-N--------------+-OBJ:V-N+         |           +MOD_ATT:+                      +-SUBJ:V-N+        +MOD_A+                          +COMP:N-V(o+          |       +MOD_ATT+             +MOD_ATT+       +MOD_ATT:N+--SUBJ:V-N-+-OBJ:V-N+-SUBJ:V-N+        +MOD_A+             |      +MOD_ATT:+        |        |        +MOD_ATT:N+---SUBJ:V-N--+           +MOD_ATT:+    
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 In a system of purified components , the affinity of binding of __SP__ __NODE__ protein and a protein protein complex consisting of cleaved __NODE__ containing a catalytic domain and of cleaved __NODE__ containing a pro domain is the same as the affinity of binding of __SP__ __NODE__ protein and a protein protein complex consisting of cleaved __NODE__ containing a pro domain and of mutant cleaved __NODE__ ( S386A ) lacking catalytic activity and containing a catalytic domain .
SUBJ:V-N (purify,system)
OBJ:V-N (purify,component)
OBJ:V-N (bind,protein)
MOD_ATT:N-ADJ (protein,__SP__)
MOD_ATT:N-ADJ (protein,__NODE__)
SUBJ:V-N (cleave,protein)
OBJ:V-N (cleave,__NODE__)
OBJ:V-N (cleave,__NODE__)
OBJ:V-N (contain,domain)
MOD_ATT:N-ADJ (domain,catalytic)
SUBJ:V-N (contain,__NODE__)
OBJ:V-N (contain,domain)
MOD_ATT:N-N (domain,pro)
COMP:N-V(of) (affinity,bind)
MOD_ATT:N-ADJ (protein,__NODE__)
MOD_ATT:N-N (protein,protein)
MOD_ATT:N-ADJ (consist,__SP__)
MOD_ATT:N-ADJ (consist,complex)
SUBJ:V-N (cleave,consist)
OBJ:V-N (cleave,__NODE__)
COMP:N-N(of) (__NODE__,__NODE__)
SUBJ:V-N (contain,__NODE__)
OBJ:V-N (contain,domain)
MOD_ATT:N-N (domain,pro)
MOD_ATT:N-ADJ (__NODE__,mutant)
MOD_ATT:N-ADJ (__NODE__,cleave)
APPOS (__NODE__,activity)
MOD_ATT:N-N (activity,S386A)
MOD_ATT:N-N (activity,lacking)
MOD_ATT:N-ADJ (activity,catalytic)
SUBJ:V-N (contain,activity)
OBJ:V-N (contain,domain)
MOD_ATT:N-ADJ (domain,catalytic)