In a system of purified components , the affinity of binding of __SP__ __NODE__ protein and a protein protein complex consisting of cleaved __NODE__ containing a pro domain and of mutant cleaved gain of function __NODE__ ( D374Y ) containing a catalytic domain is the same as the affinity of binding of __SP__ __NODE__ protein and a protein protein complex consisting of cleaved __NODE__ containing a catalytic domain and of cleaved __NODE__ containing a pro domain .