In a cell free system , binding of a DNA fragment ( 338 303 ) containing a __NODE__ response element from __SP__ __NODE__ gene and __SP__ __NODE__ protein is greater than binding of a DNA fragment ( 338 303 ) containing a __NODE__ response element from mutant __SP__ __NODE__ gene ( T 315A G 314A G 312C G 311T with its __NODE__ response element 2 mutated ) and __SP__ __NODE__ protein .